mouse anti human hla abc  (Bio-Rad)

Journal: JCI Insight

Article Title: Identification of LRP1 + CD13 + human periosteal stem cells that require LRP1 for bone repair

doi: 10.1172/jci.insight.173831

Figure Lengend Snippet: ( A and B ) Drill hole injuries were made on 12-week-old NOD scid mice ( n = 3 per group), and 5,000 LRP1 + CD13 + PDGFRA + PDPN + or 5,000 LRP1 − CD13 − PDPN − periosteal cells were FACS-sorted and transplanted. Seven and 14 days after transplantation, the transplanted cells were stained with HLA-ABC-FITC antibody and analyzed by in vivo intravital imaging. ( C and D ) On day 15 posttransplantation, transplanted LRP1 + CD13 + PDGFRA + PDPN + ( C ) and LRP1 − CD13 − PDPN − ( D ) cells were FACS analyzed for their surface expression of HLA-ABC ( E ) and SSC markers, CD13 + PDPN + ( F ), and PDGFRA + LRP1 + ( G ). ( E and F ) Graphs show the percentage of the indicated cells from C and D ( n = 3 mice per group). Data presented as mean ± SD. **P < 0.01, ***P < 0.001 by 2-tailed Student’s t test.

Article Snippet: Prior to imaging, injury sites were stained with primary mouse monoclonal anti-human HLA-ABC FITC antibody (Invitrogen, 11-9983-42, 1:20) for 20 minutes in the dark, then washed with PBS.

Techniques: Transplantation Assay, Staining, In Vivo, Imaging, Expressing

Journal: JCI Insight

Article Title: Identification of LRP1 + CD13 + human periosteal stem cells that require LRP1 for bone repair

doi: 10.1172/jci.insight.173831

Figure Lengend Snippet: ( A – E ) Drill hole injury was made on 12-week-old NSG-EGFP (NOD scid IL2Rgamma null CAG-EGFP) mouse tibia ( n = 3 per group) and 10,000 LRP1 + CD13 + periosteal cells were FACS-isolated and transplanted. Fourteen days after transplantation, animals were euthanized and tibia isolated for immunofluorescence staining for ( A – C ) HLA-ABC-PE, LRP1, ( D ) aggrecan (ACAN), and ( E ) osteocalcin (OCN) antibody. (HLA-ABC + LRP1 + , HLA-ABC + Acan + , or HLA-ABC + Ocn + cells are indicated with yellow arrow) ( F ) Hind limb 3D reconstructed μCT images of bone organoids derived from LRP1 + CD13 + P-SSCs transplanted into the gastrocnemius muscle 14 days after transplantation. ( G ) Immunostaining of xenograft bone organoids derived from LRP1 + CD13 + P-SSCs implanted into the gastrocnemius muscle of NSG-EGFP mouse hosts with indicated antibody staining (HLA-ABC + Ocn + bone ossicles are indicated with orange arrow).

Article Snippet: Prior to imaging, injury sites were stained with primary mouse monoclonal anti-human HLA-ABC FITC antibody (Invitrogen, 11-9983-42, 1:20) for 20 minutes in the dark, then washed with PBS.

Techniques: Isolation, Transplantation Assay, Immunofluorescence, Staining, Derivative Assay, Immunostaining